CLEMENS ANKLIN - VICE-PRES. BRUKER
BioSpin - TopSpin
Dr. Clemens Anklin is a Vice President of Applications and Training for Bruker and an experienced user of the TopSpin software packaged that operates Bruker spectrometers used to acquire high-resolution multi-dimensional NMR data.
Dr. Clemens received his Ph.D. from the Swiss Federal Institute of Technology (ETH Zurich) and has been with Bruker since 1988. He presented at the first NMR BootCamp in 2005 and also presented in 2006 at the first "NANUC Roadshow" in London, Ontario. This year’s focus will be on the set-up, acquisition, processing, and analysis of BioNMR experiments utilizing Bruker’sTopSpin software suite. The lecture will also include a discussion of the latest additions to the experimental library and new features. We are very pleased that he will be able to join us in Connecticut and as always we look forward to his lively presentation style.
JEFF HOCH - DIRECTOR NMR STRUCTURAL BIOLOGY FACILITY UNIVERSITY OF CONNECTICUT HEALTH CENTER
Rowland NMR ToolKit (RNMRTK)
Jeff Hoch is a professor at the University of Connecticut who specializes in the processing of linear and non-linear NMR data. His work specifically focuses on novel methods of data collection that is not limited to the use of the Fourier Transformation.
Prof. Hoch will be presenting the capabilities of the Rowland NMR ToolKit (commonly known as the RNMRTK) and will be demonstrating to students how to process rapid acquisition (i.e. non-uniform or non-linear) NMR data incompatible with traditional processing methods.
We eagerly look forward to his visit and latest developments and would like to thank Dr. Hoch for suggesting the University of Connecticut Health Center for this year's NMR BootCamp.
RYAN T.MCKAY - Scientific Director, NANUC, University of Alberta
VNMRJ and BioPack
Dr. McKay has 15 years of experience with VNMRJ and BioPack software and has been authorized by George Gray of Varian Inc. (our past presenter) to present this material. Dr. McKay has presented seminars on VNMRJ and BioPack software many times at the University of Alberta. Students will learn how to navigate the latest spectrometer operating software (2.1B and 2.2C), utilize user-friendly components, as well as perform customizations of their environments for individual requirements.
The demonstrations will then progress to the “BioPack“ software package that was designed to provide "User-friendly" water suppression, double and triple resonance 2D/3D/4D pulse sequences, including TROSY options and BioNMR PSG programming. The software features automatic calibration and subsequent experiment execution minimizing potential user error. It includes experiments for non-labeled and labeled biomolecules such as peptides, proteins, RNA and DNA's. Execution of complex sequences contributed to BioPack from large laboratories such as the Lewis Kay (Univ. of Toronto) and Ad Bax (NIH/NIDDKD) sequence libraries will be discussed.
CHARLES D. SCHWIETERS- HEAD of the STRUCTURAL BIOLOGY GROUP NIH
XPLOR-NIH
Dr. Schwieters received his B.Sc. from the University of Virginia and his Ph.D. in Chemistry from Princeton.
He develops and implements novel algorithms for probing proteinstructure and dynamics. He is responsible for the development andmaintenance of the Xplor-NIH and VMD-XPLOR software packages. Hesupervises a small group at the main NIH campus in Bethesda, MD.
2008 will be Charles' third NMR BootCamp and this year's focus will be on a more stepby-step approach to solving BioNMR problems with anemphasis on protein 3D structures and how to easily incorporate data andevaluate final solutions. Students will be presented with backgroundinformation and then given several example problems to work on. Helpwill be provided and participants should leave with a basicunderstanding and ability to utilize the program to solve their research goals.
FRANK DELAGLIO - NMR Science Inc.
NMRPipe, NMRDraw, TALOS DYNAMO
Dr. Frank Delaglio is very familiar with the NANUC training courses having presented at a specialized course in 2003 and our previous BootCamps from 2005 - 2007. We of course enthusiastically included him in this years festivities and he has again graciously volunteered to arrive early and stay late in order to answer student’s questions.
Dr. Delaglio (aka the Big D.) will be presenting the extensive software suite from Prof. Ad Bax's laboratory at the National Institutes of Diabetes and Digestive & Kidney Diseases. The software includes the well-known and widely utilized NMRPipe, NMRDraw processing tools as well as the powerful TALOS, and DYNAMO programs. The emphasis this year will be on real world examples for students to work through.
As always we look forward to Frank's detailed yet relaxed lecture style.
LEO SPYRACOPOULOS - Associate Professor University of Alberta
NMR Relaxation via Mathematica Notebooks (Wolfram Research)
Dr. Spyracopoulos is a professor in the Department of Biochemistry at the University of Alberta. The Spyracopoulos laboratory is involved in determining the dynamics, structures, and interactions of enzymes of the ubiquitination cycle, a biochemical process important in DNA repair and the immune response.
Dr. Spyracopoulos specializes in the analysis of protein dynamics through measurement of 15N NMR relaxation. He recently developed a suite of Mathematica Notebooks for analysis of protein dynamics data, which are available via his laboratories web site. The key features of the programs include ease of use, automatic visualization of data and results, and computer compatibility. The aims of the talk will be to familiarize students with analysis of protein main chain 15N dynamics using methods such as spectral density mapping and the "model-free" approach. The applicability of the methods with respect to spectral quality and sample conditions such as protein oligomerization will be discussed. Overall, students will learn how to carefully analyze protein main chain dynamics data, apply dynamics approaches to answer questions regarding biological function, and use dynamics analyses to complement protein structure determination.
BRUCE JOHNSON PRESIDENT - ONE MOON SCIENTIFIC
NMRView J
Bruce Johnson is the Founder and President of One Moon Scientific, Inc. He is the creator of NMRViewJ, one of the leading programs for the analysis and visualization of processed data on proteins and nucleic acids from NMR spectrometers.
Prior to One Moon Scientific, Inc., Bruce was a Senior Research Fellow at Merck & Co., Inc. During his 15-year career at Merck he specialized in applying the methods of structural biology, molecular modeling, and cheminformatics to research drug discovery. Bruce holds a BS degree from the University of Washington and a PhD from Duke University.
Presentations will include all the operational features of NMRViewJ (i.e. opening data, making assignments, picking NOEs for structure calculations, and exporting). Students will gain the ability to analyze spectra from multi-dimensional experiments and how to access online resources for further individual progress.
MICHAEL GRYK - Assistant Professor University of Connecticut
Connjur
Dr. Michael Gryk received his B.S. in biophysics and M.S. in chemistry from the University of Connecticut, and his Ph.D. in biophysics from Stanford University. He is currently an assistant professor at the University of Connecticut who specializes in both bioinformatic analysis of proteins as well as using NMR to study protein structure and dynamics. He is currently collaborating with researchers at Trinity College, Hartford, CT to develop a software integration platform for biomolecular NMR analysis.
Prof. Gryk will be presenting the design and functionality of this newly-developed software integration platform, called CONNJUR. CONNJUR provides an integration platform for nmrPipe and the Roland NMR Toolkit and facilitates processing schemes in which a mixture of the software packages is desired.